Clinical Medicine Laboratory

The Clinical Medicine Laboratory (CML) tailor and explores considering animal health care practice aiming to recuperate from pathological states to achieve optimum health management vis-à-vis amelioration. It may include in vitro/ in vivo (laboratory animals)/ in vivo (clinical cases) work. Deviations from normalcy may involve clinical, haematological, biochemical, oxidant-antioxidants, tissue, organ, organ systems. Ameliorative is evaluated and the better one is identified.

Thematic area of lab

Address alterations during healthy vis-à-vis pathological states vis-a-vis identifying optimum amelioration

Infrastructure and facility in lab

• Refrigerator
• Centrifuge machine
• Deep Fridge
• Microscope
• Weighing Balance
• Incubator
• Hot air oven

Major Achievement of Lab

• Ageing is associated with significant (P<0.05) changes in oxidative stress indices in dogs and cattle.
• Benign prostatic enlargement, focal gastric thickening and fatty degenerative changes in liver are predominant ultrasound changes in 8-10 year age group and > 10 year age group dogs.
• Radiographic findings in dogs suggest spondylotic changes, degenerative changes in hip joint and osteoarthritic changes were more evident in 8-10 year age group and > 10 year age group dogs.
• Ascorbic acid was found to be effective in ameliorating oxidative stress and altered haematological parameters in both dogs and cattle

List of projects going on in lab

• Explore and tailor for safeguarding health management addressing ageing for healthy longevity (Start: 1.11.2022; End Date: 30.4.2024 ; Funding Agency: ICAR- IVRI

Name and contact details of Lab in-charge

• Dr Umesh Dimri Principal Scientist E-mail: udimrimesh@rediffmail.com

Comaparative System of Medicine (CSM) Lab

Comparative System of Medicine laboratory is well equipped laboratory to undertake research in biomedical field to explore alternative sources for healthcare of animal. The current work of this laboratory mainly focussed to scientific validation of the efficacy and safety of medicinal plants for rationalization of their use as alternate medicine. Infrastructure to carryout research for separation and purification of active components from medicinal plants, their biological testing for efficacy both in vitro and in vivo system against various infectious (Trypanosoma evansi ) and non-infectious diseases ( renal, osteoskeletal system and heavy metal and fluoride toxicity) using various chromatographic techniques, cell culture, Atomic absorption Spectrophotometry, ion selective potentiomentry, haematology and clinical biochemistry.

Thematic area of lab

• Activity guided separation and purification of secondary plant metabolites
• Evaluation of efficacy and safety of purified as well as pure compounds for wound healing ,antitrypanosomal, antiurolithiatic, renoprotective potential to develop herbal remedy of these conditions of animals

Infrastructure and facility in lab

• HPTLC
• Atomic Absorption spectrophotometer
• Ion selective Potentiometer
• ELISA Reader
• CO2 incubator
• Inverted Microscope
• Refrigerated centrifuge
• BSL-2 cabinet
• Haematology analyzer
• Spectrophotometer

Major Achievement of Lab

• Scientifically validated 26 medicinal plants used by ethnic peoples for renal disorder, antitrypanosomal antidiabetic heavy metal toxicity
• Developed an herbal product to antagonize fluoride toxicity
• Isolated and identified 14 pure molecules (in collaboration with IICT,Hyderabad) few of these molecules possess promising activities
• Published research findings in high impact journals such as science of the total environment, Ecotoxicology and Environmental safety, Toxicology, Journal of Ethnopharmacology, Biomedicine and Pharmacotherapy etc
• Work of this laboratory cited in text book such as Veterinary Medicine by Radostits and co-workers
• The laboratory provided bench places to more than 42 PG students for their thesis work
• Developed 2 patents/products {OLINAL (Skin ointment) and FLUORICURE: herbal remedy for fluoride toxicity)

List of projects going on in lab

• All India Network Programme on Ethnoveterinary Medicine

Name and contact details of Lab in-charge

• Dr Sahadeb Dey Principal Scientist Division of Medicine ICAR-Indian Veterinary Research Institute Izatnagar 243122 Mail.id: dahadeb.dey@icar.gov.in

Metabolic Disease Lab

Metabolic Disease laboratory is well equipped laboratory to undertake research in biomedical field to explore metabolic diseases of animal healthcare. The remarkable achievements of this laboratory for the increased productivity of livestock and betterment of companion animal have been accomplished form time to time. Clinical and nutritional management of gastro-intestinal disorders in livestock with special reference to acute diarrhoea in neonates and young ones has been attained. Technology oriented programme attained by using bovine colostrum to supplement proven therapeutic potential in ailing animals. Research work accomplished for development of sensitive diagnostic marker to institute early therapy and development of catalytic therapy for better management of liver disorders. Also developed cost effective sustainable herbal acaricide to control ticks Current work in this laboratory mainly focussed to scientific research on hepato-renal biomarkers in canine babesiosis and hepatoprotectant effects of certain bioorganics; Diagnostic tests for Babesia gibsoni & its hepatic involvement with special reference to therapeutic management; Hepatoprotective efficacy of nano-structured catechin encapsulated biopolymer in lab animal; bone marrow stimulating property of some bio chemicals for therapeutic management of aplastic pancytopenia associated with canine hemoprotozoan diseases etc. using various modern techniques and lab instruments of clinical importance.

Thematic area of lab

• Improved production oriented research for the betterment of livestock and companion animal.

Infrastructure and facility in lab

• UV-VIS spectrophometer
• Distillation appartus
• Magnetic stirrer
• Centriguge
• Microscope
• Soxhlet appatus

Major Achievement of Lab

• Package of practices developed for prevention and management of diarrhoea in calves
• Keeping quality of bovine colostrum enhanced excessively by lyophilization without any significant loss in the IgG immunoglobulin concentration paving way for establishment of colostral bank.
• Herbo-mineral drug formulation as a component of integrated tick control measures in bovines
• Nano-structured catechin encapsulated biopolymer as hepatoprotectant in lab animal;

List of projects going on in lab

• CCRH (Min. of AYUSH) funded Research Project on ‘Potential of homeopath drugs against neonatal calf diarrhoea’

Name and contact details of Lab in-charge

• Dr. D B Mondal, PS, Division of Medicine E-mail: dbmondal@gmail.com

PG Lab cum CIF

This is dedicated facility for post-gradute students research. Besides, the facility is open for post gradute student of other divisions of Institute. This laboratory offer basic facility of research to all the students and specialized facility related to research in area of nanodrug delivery and nanotherapeutics.

Thematic area of lab

• Nanotherapeutics

Infrastructure and facility in lab

• Incubator
• High precision digital weighing balance
• Centrigufe machine
• Refrigerator
• Deep freez
• Hot plate
• Digital pH meter
• Serological water bath
• Verticle Franz Drug Diffusion test apparatus

Major Achievement of Lab

• Ethosome capped C. longa was found to have better wound healing capacity in comparison to crude extract.
• The MIC of LGE was tested on 67 clinical microbial isolates. The lowest MIC was found as 0.008 µg/ml. against (Bacillus stearothermophilus , Enterobacter agglomerans, Escherichia coli, Staphylococcus lentus and Streptococcus milleri) wheras the highest MIC was recoded as 8 mcg/ml against Alcaligenes faecalis, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris. Average zone of inhibition recorded against 147 microbial isolates showed concentration dependent increase in ZI viz. 12.5 µg/ml (30.1 mm) and at 25 µg/ml (33.5 mm).
• The GOE at concentration of 25 µg of GO/ml was found effective against all tested isolates (147 nos) whereas at concentration of 12.5 µg/ml was ineffective against only one isolate of Aspergillus niger out of 147 isolates tested. The GOE tested at 6.25 µg/ml inhibited 139 isolates out of 147 whereas failed to inhibit Bacillus cereus, Paenibacillus alvei (n=2 isolates), Staphylococcus delphini, Aeromonas trota, Aspergillus niger, Enterococcus faecalis and Enterobacter agglomerans.
• The MIC values remains fairly constant against Staph. aureus (ATCC 29312 and ATCC 43300) and E .coli (NDM)at day 0 and up to 265 day of testing on storage at room temp.
• MBC values against majority of isolates were in range of 125-250 µg/ml. The lowest value of MBC (3.97 µg/ml) was against Klebsiella 2238HNSP whereas the highest MBC (500 µg/ml) was against E.coli and Streptococcus pyogens isolates.
• The MFC crude oil was 39 µg/ml against M. canis and T. mentagrophytes whereas the MFC of ethosomal suspensions ethosomes was 78µg/ml and 156 µg/ml respectively against M. canis and T. mentagrophytes.
• The in vitro release kinetics showed that GOE is sustained released formulation (Zero-order) with perfect fitting with Korsemeyer Peppas and Weibull release models designed for studying the release of drug from polymeric matrix.
• Comparative study of oral and injectible iron therapy with or without ascorbic acid co-administration in piglet anemia demonstrated that both oral and injectible iron indcued severe oxidative stress post treatment. Co-administration of ascobic acid was found effective in alleviating the oxidative stress along with better body weight gain , Hb, N;L ratio and Ig level.
• Comparative study of nano iron formulation with Asc-INOP and FA- INOP suggested better therapeutic potential of Asc & FA INOPs as compared to bare INOP and conventional ferruos sulphate.

List of projects going on in lab

• Formulation and evaluation of Ethosomes a Novel trans-dermal delivery system for enhanced antimicrobial and antidermatophytic effect of essential oils (ongoing)

Name and contact details of Lab in-charge

• Dr. Akhilesh Kumar Scientist (SS) Email: dr_akhil2005@yahoo.co.in Mob no: +91-8794741191

Preventive Medicine Lab

Disease prevention is an essential component of comprehensive veterinary medical care. The Preventive Medicine Laboratory is well equipped laboratory to undertake research for the development of methods of disease control and prevention and promote health in human and animal population. The current work of this laboratory mainly focussed to research related to diagnosis and prevention of gastrointestinal disorders in animals. Infrastructure to carryout research for determining the main etiology of gastrointestinal disorders using microbial culturing techniques and culture sensitivity testing to develop the effective control measures.

Thematic area of lab

• Address Gastrointestinal related disorders in animals and mastitis

Infrastructure and facility in lab

• Refrigerator
• Centrifuge machine
• Deep Fridge
• Microscope
• Weighing Balance
• Incubator
• Hot air oven

Major Achievement of Lab

• Therapeutic potential of polymixin E (colistin) in combating the endotoxemia in haemorrhagic gastroenteritis due to canine parvovirus infection in puppies.
• Combination of pantoprazole, silymarin, vit. C and carvedilol as an effective combination therapy for the therapeutic management of hepatic origin secondary gastritis in canines.
• Formulated polyherbal teat dip for matitis

List of projects going on in lab

• Refining of diagnostic and therapeutic protocol for secondary gastritis in canines (Principal Investigator; Start date: Sept, 2019 Aug, 2021, Funding Agency: ICAR-IVRI)

Name and contact details of Lab in-charge

• Dr S.K.Dixit Principal Scientist E-mail: shivendradixitivri@gmail.com