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Major Achievements

  • An indigenous method called “Tupol Technique” for freezing bull semen.
  • A new staining technique to stain different parts of spermatozoa.
  • Technique of separation of “Sperm Acrosomal Cap” by chemical method.
  • Modified protocol for buffalo semen freezing.
  • Enhanced conception rate in cows with ascorbic acid supplemented semen.
  • Purification and characterization of PDC-109, a fertility related protein from seminal plasma of buffalo bull.
  • Reduction of sperm cryo-injury using non-heparin binding protein
  • Improved freezability of cattle & buffalo semen by reducing oxidative stress through partial deoxygenation of extender, supplementation with cholesterol loaded cyclodextrine and nano-purification of semen.