Indian Veterinary Research Institute
भारतीय पशुचिकित्सा अनुसंधान संस्थान

Bacteriology & Mycology Division

Major Achievements

  • A low volume saponified vaccine against Haemorrhagic Septicaemia has been developed and found effective against challenge infection.
  • A status of freedom from Contagious bovine pleuropneumonia infection in cattle & buffalo was obtained from OIE.
  • A diagnostic kit for Caprine Pleuropneumonia has been developed and found satisfactory for field diagnosis.
  • A double deletion mutant of Salmonella Abortus equi was developed as a vaccine strain and found effective in controlling contagious abortion in mares.
  • Diagnostically important protein genes of Leptospira interrogans were produced using recombinant DNA technology and successfully used for serodiagnosis of leptospirosis in animals.
  • A saponified sonicate killed vaccine using Mycoplasma mycoides subsp. capri against Caprine Pleuropneumonia has been developed and found protective upto 1 year.
  • Molecular characterization of various mycoplasmal species has been carried out with the help of SDS-PAGE, western blotting, restriction endonuclease analysis for accurate and quick diagnosis of mycoplasma.
  • Various PCR, viz; mycoplasma specific, mycoide cluster specifics, mycoides group specific, RAPD-PCR and multiplex PCR were standardized for quick detection and differentiation of different species of mycoplasma.
  • Direct detection of mycoplasma from tissue materials and nasal strips with the help of PCR was made possible.
  • Molecular identification of Pasteurella multocida by using PM-PCR, HSB-PCR, Nested PCR, Multiplex-PCR, RAPD-PCR, REP-PCR and RFLP assays.
  • The recombinant L7/L12 ribosomal protein of Brucella abortus and its DNA vaccine were found to be completely safe in pregnant guinea pigs.
  • Several Salmonella serovars, namely Billa, Mons, Muenchen, Reinckandorf, Starrenbos, Uganda, Wein, S.I.17:d:1,2, S.1.17:d (Monophasic) were identified for the first time in India.
  • Molecular typing for Indian strains of Salmonella using RAPD, RFLP, AFLP, AP PCR, ERIC PCR, IS 200 and 16S rrn lock polymorphism.
  • PCR assays using pncA and oxyR and 500 bp genes for species identification of Mycobacterium tuberculosis and Mycobacterium bovis was standardized.
  • Alternaria sp. and Moticrella wolfii were reported first time in India and the infection due to P.roseures, R.hylatinispora Gymnoascoides, G.citrinia & Ch. tropicum was established.